The Felis_catus-6.2 assembly of the cat (Felis catus) genome was produced by the International Cat Genome Sequencing Consortium. The whole assembly is composed of 19 chromosomes and plasmids, 6399 scaffolds with a N50 value of 4.7Mb and 203082 contigs with a N50 value of 20.6kb.
The genome assembly represented here corresponds to GenBank Assembly ID GCA_000181335.2
Felis_catus-6.2 was annotated using a standard Ensembl mammalian genebuild pipeline, incorporating RNA-seq data provided by The Texas A&M College of Veterinary Medicine & Biomedical Sciences, Department of Veterinary Integrative Biosciences. The final gene set is made up from 19493 protein coding genes, 542 pseudogenes, and 1855 non-coding RNA genes.
RNASeq data set
As part of the main gene set, we have predicted gene models based on RNA-seq data from testis tissue of four individuals (unpaired 50bp reads) using the RNA-Seq pipeline. We did similarity matching (BLASTp) of these models against UniProt proteins having evidence of existence on protein or transcript level (protein existence level 1 and 2) in order to confirm the open reading frame (ORF). A total of 3504 RNA-seq transcript models were used in the final gene set. The data was also used to add UTR to gene models.
In addition to the main gene set, the Ensembl RNA-seq pipeline built the following numbers of gene models for each of the four individuals and for the merged data set:
|Individual||Number of gene models|
General information about this species can be found in Wikipedia.
|Assembly||Felis_catus_6.2, INSDC Assembly GCA_000181335.2, Sep 2011|
|Golden Path Length||2,455,541,136|
|Genebuild method||Mixed strategy build|
|Genebuild started||Dec 2011|
|Genebuild released||Jan 2013|
|Genebuild last updated/patched||Jan 2013|
Genes and/or transcript that contains an open reading frame (ORF).
|Small non coding genes|
Small non coding genes are usually fewer than 200 bases long. They may be transcribed but are not translated. In Ensembl, genes with the following biotypes are classed as small non coding genes: miRNA, miscRNA, rRNA, tRNA, scRNA, snlRNA, snoRNA, snRNA, tRNA, and also the pseudogenic form of these biotypes. The majority of the small non coding genes in Ensembl are annotated automatically by our ncRNA pipeline.
A pseudogene shares an evolutionary history with a functional protein-coding gene but it has been mutated through evolution to contain frameshift and/or stop codon(s) that disrupt the open reading frame.
|Gene transcriptsNucleotide sequence resulting from the transcription of the genomic DNA to mRNA. One gene can have different transcripts or splice variants resulting from the alternative splicing of different exons in genes.||22,656|
|Genscan gene predictions||45,433|