After the previous zebrafish assembly (Zv9) was released in July 2010, the zebrafish genome sequence was given into the care of the GRC for future improvement and maintenance. The GRC have recently produced GRCz10, which is the tenth zebrafish reference assembly.
The previous assembly (Zv9), although of high quality, featured many gaps and suffered from sub-optimal long-range continuity. In order to overcome this, the GRC have sequenced more than 1500 additional BAC and fosmid clones and added them to the assembly.
The most notable changes in the chromosome landscape since the previous assembly can be found on chromosome 4, which has gained about 15 Mb in length. Also, 94 of 112 previously unplaced clone-contigs have now been placed on a chromosome. The current assembly contains a total sequence length of 1.37Gb with 2.09Mb of gaps. There are 26 chromosomes (including the mitochondrion) and 3,399 scaffolds, composed of 22,852 contigs with a scaffold N50 of 2.18Mb and a contig N50 of 1.232Mb. The N50 size is the length such that 50% of the assembled genome lies in blocks of the N50 size or longer.
The genome assembly represented here corresponds to GenBank Assembly ID GCA_000002035.3
The Ensembl GRCz10 assembly was annotated using Ensembl's automatic annotation pipeline. Predictions from zebrafish proteins have been given priority over predictions from other non-mammalian vertebrate species. All Uniprot proteins were filtered to remove predictions (PE level 3 and above). Aligned zebrafish cDNAs and zebrafish RNASeq data have been used to add UTRs. RNASeq data from embryonic and olfactory epithelium tissues were also used to produce gene models. Genes are named based on the alignment of their coding regions to known entries in public databases; ZFIN genes have priority in this process.
The Ensembl annotations were then merged with Vega annotations at the transcript level. Transcripts were merged if they shared the same internal exon-intron boundaries (i.e. had identical splicing pattern) with slight differences in the terminal exons allowed. Importantly, all Vega source transcripts (regardless of merge status) were included in the final merged gene set.
Additional manual annotation of this genome can be found in Vega
General information about this species can be found in Wikipedia.
|Assembly||GRCz10 (Genome Reference Consortium Zebrafish Build 10), INSDC Assembly GCA_000002035.3, Sep 2014|
|Golden Path Length||1,371,719,383|
|Genebuild method||Mixed strategy build|
|Genebuild started||Sep 2014|
|Genebuild released||May 2015|
|Genebuild last updated/patched||Nov 2016|
|Coding genes||25,832 (incl 48 ) readthrough|
|Non coding genes||6,060|
|Small non coding genes||3,173|
|Long non coding genes||2,792 (incl 8 ) readthrough|
|Misc non coding genes||95|
|Genscan gene predictions||36,087|