This is the first release of the low-coverage 2.05X assembly of the two-toed sloth (Choloepus hoffmanni). The genome sequencing and assembly is provided by the Broad Institute.
The N50 size is the length such that 50% of the assembled genome lies in blocks of the N50 size or longer. The contigs have an N50 length of 2461 bp and 8.6 kb for supercontigs. The total number of bases in supercontigs is 2.34 Gb and in contigs is 1.95 Gb.
The project coordination and genome sequencing and assembly is provided by the Human Genome Sequencing Center at Baylor College of Medicine
Owing to the fragmentary nature of this preliminary assembly, it was necessary to arrange some scaffolds into "gene-scaffold" super-structures, in order to present complete genes. There are 8139 such gene-scaffolds, with identifiers of the form "GeneScaffold_1".
Mammalian Genome Project
Choloepus hoffmanni is one of 24 mammals that will be sequenced as part of the Mammalian Genome Project, funded by the National Institutes of Health (NIH). A group of species were chosen to maximise the branch length of the evolutionary tree while representing the diversity of mammalian species. Low-coverage 2X assemblies will be produced for these mammals and used in alignments for cross-species comparison. The aim is to increase our understanding of functional elements, especially in the human genome.
General information about this species can be found in Wikipedia.
|Assembly||choHof1, Sep 2008|
|Golden Path Length|
The golden path is the length of the reference assembly. It consists of the sum of all top-level sequences in the seq_region table, omitting any redundant regions such as haplotypes and PARs (pseudoautosomal regions).
|Genebuild method||Projection build|
|Genebuild started||Nov 2008|
|Genebuild released||Feb 2009|
|Genebuild last updated/patched||May 2010|
Genes and/or transcript that contains an open reading frame (ORF).
|Small non coding genes|
Small non coding genes are usually fewer than 200 bases long. They may be transcribed but are not translated. In Ensembl, genes with the following biotypes are classed as small non coding genes: miRNA, miscRNA, rRNA, scRNA, snlRNA, snoRNA, snRNA, and also the pseudogenic form of these biotypes. The majority of the small non coding genes in Ensembl are annotated automatically by our ncRNA pipeline. Please note that tRNAs are annotated separately using tRNAscan. tRNAs are included as 'simple fetaures', not genes, because they are not annotated using aligned sequence evidence.
A pseudogene shares an evolutionary history with a functional protein-coding gene but it has been mutated through evolution to contain frameshift and/or stop codon(s) that disrupt the open reading frame.
|Gene transcriptsNucleotide sequence resulting from the transcription of the genomic DNA to mRNA. One gene can have different transcripts or splice variants resulting from the alternative splicing of different exons in genes.||16,102|
|Genscan gene predictions||109,168|