Callithrix jacchus-3.2.1 (GCA_000004665.1) is a draft assembly of the common marmoset genome. This assembly is used by UCSC to create their calJac3 database. It was sequenced to 6X coverage using a female marmoset (animal id #186/17066) DNA source kindly provided by Dr. Suzette Tardif, of the Southwestern National Primate Research Center (San Antonio, Texas). The DNA source for the CHORI-259 BAC library was male and the full brother of the female used for whole genome shotgun sequencing (#186/17066). The combined sequence reads were assembled using the PCAP software (Genome Res. 13(9):2164-70 2003) and filtered for all known non-marmoset sequence contaminants.
The new Callithrix jacchus-3.2.1 assembly spans 2.75Gb with 2.62Gb ordered and oriented along 24 chromosomes. Chromosomes were not available in Callithrix jacchus-3.2 (released in Ensembl 56 and Ensembl 57), which was composed of scaffolds and contigs only.
The N50 length is defined as the scaffold (or contig) length N for which 50% of the genome lies in scaffolds (or contigs) of the N50 size or longer. The Callithrix jacchus-3.2.1 assembly is made up of a total of 73,756 scaffolds with an N50 scaffold length of over 6.4Mb. Scaffolds placed on chromosomes have been annotated as described below.
The sequence assembly was provided by The Genome Center, Washington University School of Medicineand the Human Genome Center, Baylor College of Medicine.
The genome assembly represented here corresponds to GenBank Assembly ID GCA_000004665.1
The gene set for marmoset was built using the Ensembl gene annotation pipeline. Species-specific resources for marmoset are relatively limited and therefore sequences from other primates, especially human, were also used as supporting evidence for coding transcript models. In addition to the coding transcript models, non-coding RNAs and pseudogenes were annotated. Marmoset cDNAs and ESTs and human cDNAs were mapped to the genome using Exonerate.
General information about this species can be found in Wikipedia.
|Assembly||C_jacchus3.2.1, INSDC Assembly GCA_000004665.1, Jan 2010|
|Golden Path Length|
The golden path is the length of the reference assembly. It consists of the sum of all top-level sequences in the seq_region table, omitting any redundant regions such as haplotypes and PARs (pseudoautosomal regions).
|Genebuild method||Full genebuild|
|Genebuild started||Feb 2010|
|Genebuild released||May 2010|
|Genebuild last updated/patched||Feb 2014|
Genes and/or transcript that contains an open reading frame (ORF).
|Small non coding genes|
Small non coding genes are usually fewer than 200 bases long. They may be transcribed but are not translated. In Ensembl, genes with the following biotypes are classed as small non coding genes: miRNA, miscRNA, rRNA, scRNA, snlRNA, snoRNA, snRNA, and also the pseudogenic form of these biotypes. The majority of the small non coding genes in Ensembl are annotated automatically by our ncRNA pipeline. Please note that tRNAs are annotated separately using tRNAscan. tRNAs are included as 'simple fetaures', not genes, because they are not annotated using aligned sequence evidence.
A pseudogene shares an evolutionary history with a functional protein-coding gene but it has been mutated through evolution to contain frameshift and/or stop codon(s) that disrupt the open reading frame.
|Gene transcriptsNucleotide sequence resulting from the transcription of the genomic DNA to mRNA. One gene can have different transcripts or splice variants resulting from the alternative splicing of different exons in genes.||55,116|
|Genscan gene predictions||53,363|