The UMD 3.1 assembly (NCBI assembly accesion GCA_000003055.3), released in December 2009, is the third release of the cow (Bos taurus) assembly from the Center for Bioinformatics and Computational Biology (CBCB) at University of Maryland. The genome sequences were generated using a combination of BAC-by-BAC hierarchical (~11 million reads) and whole-genome shotgun (~24 million reads) sequencing methods, assembled using the Celera Assembler version 5.2.
The total length of the UMD3.1 assembly is 2.65Gb. The N50 size is the median sequence length, i.e. 50% of the assembled genome lies in blocks of the N50 size or longer. The N50 size for contigs in the UMD3.1 assembly is 103785.
The genome assembly represented here corresponds to GenBank Assembly ID GCA_000003055.3
Gene annotation in cow has been generated by alignment of proteins from three major sources (in descending order of their contribution to the final gene set):
- Bos taurus (cow) proteins from UniProtKB and NCBI RefSeq and Bos taurus cDNAs from INSDC. For proteins, UniProtKB proteins were filtered to keep only those of protein existence (PE) levels 1 and 2 (i.e. with protein or transcript level evidence), and only curated RefSeq proteins (accessions starting with "NP_") were used. For cDNAs, they were also filtered to keep curated RefSeq mRNAs (accessions starting with "NM_") and to remove those associated with PE level 3-5 UniProt proteins.
- UniProt mammalian and vertebrate proteins of PE levels 1 and 2; and
- Translations of Ensembl human genes which have one-to-one orthologues in mouse (taken from Ensembl release 62 data).
General information about this species can be found in Wikipedia.
|Assembly||UMD3.1, INSDC Assembly GCA_000003055.3, Nov 2009|
|Golden Path Length||2,670,422,299|
|Genebuild method||Full genebuild|
|Genebuild started||Apr 2011|
|Genebuild released||Sep 2011|
|Genebuild last updated/patched||Sep 2011|
Genes and/or transcript that contains an open reading frame (ORF).
|Small non coding genes|
Small non coding genes are usually fewer than 200 bases long. They may be transcribed but are not translated. In Ensembl, genes with the following biotypes are classed as small non coding genes: miRNA, miscRNA, rRNA, tRNA, scRNA, snlRNA, snoRNA, snRNA, tRNA, and also the pseudogenic form of these biotypes. The majority of the small non coding genes in Ensembl are annotated automatically by our ncRNA pipeline.
A pseudogene shares an evolutionary history with a functional protein-coding gene but it has been mutated through evolution to contain frameshift and/or stop codon(s) that disrupt the open reading frame.
|Gene transcriptsNucleotide sequence resulting from the transcription of the genomic DNA to mRNA. One gene can have different transcripts or splice variants resulting from the alternative splicing of different exons in genes.||26,740|
|Genscan gene predictions||47,100|