News for Chinese hamster CriGri Ensembl Release 90

News categories

New web displays and tools

Scaling custom tracks

In this release we have added the ability to adjust the y-axis of your "wiggle" tracks, such as BigWig and bedGraph files. This feature is available in two places on the website:

  1. When you upload a bedGraph file or attach a BigWig, you will have the option to set the minimum and maximum values for the y-axis (as well as the colour of the graph)
  2. Once the track is displayed, you will be able to change the values by clicking on the track name in the left-hand edge of the image and using the popup menu - the tab is marked by a histogram icon (see screenshot, below).

Disconnecting and reconnecting track hubs

Many track hubs have a lot of tracks, and if you want to configure which tracks are shown it can take quite a while. And if you delete the hub from your Ensembl session and then add it back, you have to go through the whole process all over again!

To save time and effort, you can now disconnect the track hub from the browser and then reconnect it later without losing your configuration changes. In the 'Your Data' table there is now a disconnect/reconnect icon - just click on it and then close the control panel to update the genome browser.

Note that if you want to be able to reconnect a hub with the same settings whilst on a different computer, you will need to save the configuration to your Ensembl account.

New species, assemblies and genebuilds

Mouse: updated cDNA alignments

A new cdna database will be created for e89: The latest set of cDNAs for mouse (as of April 2017) from the European Nucleotide Archive and NCBI RefSeq will be aligned to the current genome using Exonerate.

New regulation data

New probe mapping data for five new rodents

Probe mappings for five new rodents are available in release 90.

Other updates


ncRNAtrees and homologies

  • Classification based on Rfam models (v12.1)
  • Multiple sequence alignments with Infernal
  • Phylogenetic reconstruction using RAxML
  • Phylogenetic reconstruction using FastTree2 and ExaML for very big families
  • Additional multiple sequence alignments with Prank (w/ genomic flanks)
  • Additional phylogenetic reconstruction using PhyML and NJ
  • Phylogenetic tree merging using TreeBeST
  • Per family gene dynamics using CAFE
  • Homology inference
  • Secondary structure plots

patch_89_90_a.sql - Schema version update

89 -> 90

Protein Families

Updated HMM families including all Ensembl transcript isoforms (including human non-reference haplotypes) and newest Uniprot Metazoa.

 -- Clustering by PantherScore (based on Ensembl HMM library)
 -- Multiple Sequence Alignments with MAFFT (v.7.221)

ProteinTrees and homologies

GeneTrees (protein-coding) with new/updated genebuilds and assemblies

 -- all-vs-all blastp (ncbi-blast-2.2.30+)
 -- Clustering using hcluster_sg
 -- Multiple sequence alignments using MCoffee (Version_9.03.r1318) or Mafft (mafft-7.221)
 -- Phylogenetic reconstruction using TreeBeST
 -- Homology inference
 -- Pairwise gene-based dN/dS scores for high coverage species pairs only (both on orthologues and paralogues) (codeml/PAML v4.3)
 -- GeneTree stable ID mapping
 -- Per family gene dynamics using CAFE (v2.2)

 -- computation of pairwise gene-order conservation score

 -- comparison of orthologies with whole-genome alignments

 -- high-confidence calls

 -- use of cd-hit to remove redundancy in blast db

patch_89_90_e.sql - new seq_member_projection and seq_member_projection_stable_id table

  • To hold information about projected members
  • Required by mouse strains and cd-hit implementation
  • allows for a representative sequence to be used in both blast and HMM classification and its resultant data to be projected onto other members of the cluster for expediency

S.scrofa alignments

We will compute LastZ alignments for new pig assembly:

  • pig v cow
  • pig v sheep
  • human v pig
  • mouse v pig

Recompute multiple alignments

EPOs containing updated species should be recomputed:

  • mammals

Pecan alignments:

  • amniotes (only to update new assemblies)

Mouse alignments

We will compute LastZ alignments for new mice, mus caroli and mus pahari

  • caroli v mouse
  • pahari v mouse
  • caroli v human
  • pahari v human

For consistency we will also align mus spretus against the reference mouse

patch_89_90_b.sql - DB Schema update:genomic_align_tree.parent_id

allow NULL in genomic_align_tree.parent_id

patch_89_90_c.sql - Mark constrained_element.p_value as NOT NULL

This will also fix a bug in the code whereby pvalue=0 was stored as NULL. From now on, p-values are always defined and 0 will be used

patch_89_90_d.sql - Allow NULL in genomic_align_tree.left_node_id/right_node_id

NULL was previously not allowed and led to 0 being used

patch_89_90_f.sql - Add missing biotype-groups in the gene_member table

The list of biotype groups was missing a few ones like "pseudogene", but also other genes that are meant to be ignored but need to be loaded for consistency

LastZ alignments: rodent collection

All the new rodents will be aligned against human and mouse with LastZ

Chinese Hamster Ovary Cell Line alignments

We will import the alignment with mouse computed by Eagle Genomics and will compute the alignment against human


External database references update

Xrefs updates for: homo_sapiens (human), mus_musculus (mouse), rattus_norvegicus (rat), sus_scrofa (pig)


Schema patches

patch_89_90_a.sql - Update schem_version in meta table to 90

patch_89_90_b.sql - Track the sequence type (genomic or a transcript) and id (seq region name or stable id) of the sequence on which a probe feature was found

patch_89_90_c.sql - Add stable id index to the probe_transcript table to speed up generation of the transcript pages on the website

patch_89_90_d.sql - Add stable id index to the probe_set_transcript table


Annotation of Chinese hamster ovary cell line assembly

We have produced gene annotation for Chinese Hamster ovary cell line assembly


Ensembl 90 mart databases

  • Ensembl Genes 90
    • Addition of new rodents, Mus Caroli, Pahari and Spretus
    • Addition of new versioned stable ID filter/attribute for Gene, Transcript and Translation
    • Retired Status filters and attributes
  • Mouse Genes 90
    • Moved Mus Spretus to Ensembl Genes mart 90
    • Retired Status filters and attributes
  • Ensembl Variation 90
  • Ensembl Regulation 90

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