The CanFam3.1 assembly of the dog genome was produced in September 2011 by the Broad Institute of MIT and Harvard. It consists of 39 chromosomes (1-38 and X) and 15 unplaced scaffolds.
The genome assembly represented here corresponds to GenBank Assembly ID GCA_000002285.2
CanFam3.1 was annotated using a standard Ensembl mammalian genebuild pipeline, incorporating RNA-Seq data provided by the Broad Institute. The annotation process is described in the document below.
RNASeq data set
In addition to the main set, we have predicted gene models for each tissue type using the RNA-Seq pipeline. We did a BLASTp of these models against UniProt proteins of protein existence level 1 and 2 in order to confirm the open reading frame (ORF). The best BLAST hit is displayed as a transcript supporting evidence. 8,968 gene models were made exclusively from RNASeq data. The data was also used to add UTR to gene models.
The tissue-specific sets of transcript models built using our RNAseq pipeline are as follows:
|Tissue||Number of gene models|
Additional manual annotation of this genome can be found in Vega
General information about this species can be found in Wikipedia.
|Assembly||CanFam3.1, INSDC Assembly GCA_000002285.2, Sep 2011|
|Golden Path Length||2,410,976,875|
|Genebuild method||Full genebuild|
|Genebuild started||Nov 2011|
|Genebuild released||Jul 2012|
|Genebuild last updated/patched||Jul 2012|
Genes and/or transcript that contains an open reading frame (ORF).
|Small non coding genes|
Small non coding genes are usually fewer than 200 bases long. They may be transcribed but are not translated. In Ensembl, genes with the following biotypes are classed as small non coding genes: miRNA, miscRNA, rRNA, tRNA, scRNA, snlRNA, snoRNA, snRNA, tRNA, and also the pseudogenic form of these biotypes. The majority of the small non coding genes in Ensembl are annotated automatically by our ncRNA pipeline.
A pseudogene shares an evolutionary history with a functional protein-coding gene but it has been mutated through evolution to contain frameshift and/or stop codon(s) that disrupt the open reading frame.
|Gene transcriptsNucleotide sequence resulting from the transcription of the genomic DNA to mRNA. One gene can have different transcripts or splice variants resulting from the alternative splicing of different exons in genes.||29,884|
|Genscan gene predictions||46,687|